Table 5 Oligonucleotide primers used in this study
Primer | Sequence (5' to 3') | Amplicon size (bp) | A.T. (°C) | Target |
|---|---|---|---|---|
1967-H9F | GGCGCACCTCAATTTAAC | 157 | 50 | 1967-H9 (Cju35) |
1967-H9R | TCCATGTTCTTCTCTCAG | Â | Â | [dmsB] |
1967-C2F | GCCTTAGGGATTGCTACA | 172 | 50 | 1967-C2 |
1967-C2R | CTTATGCTCCATTTAGGC | Â | Â | Â |
264F2 | ACTCACGTATCTTGCTTC | 859 | 50 | Cj0264c |
264R2 | ACTCGCTGCATTTTGAAG | Â | Â | Â |
629-C10F | CAAGTATTATGGCCGGAA | 459 | 50 | 629-C10 (Cju05) |
629-C10R | ATCCTATAACACTCGCAC | Â | Â | [cytC locus] |
629-D8F | AACTACGGATCCACCTACG | 339 | 50 | 629-D8 |
629-D8R | AGCGGGGTGTATTTTGGAG | Â | Â | Â |
629-D9F2 | TTGAGTAAAACGACAGC | 249 | 50 | 629-D9 |
629-D9R | TGGCCTTAGAGCTATGGA | Â | Â | Â |
961-A9F | CCAAATCCTCAGGTGTTC | 326 | 55 | 961-A9 |
961-A9R | ACGAGGTTTAGATGGTTC | Â | Â | Â |
670-D7F | TACTGATAATCCGCATTGC | 414 | 50 | 670-D7 |
670-D7R | CTTGCATATTCACCCCTT | Â | Â | Â |
FlaA1 | GCATTTCGTATTAACACACAAATGGTGC | 1700 | 55 | flaA |
FlaA2 | CTGTAGTAATCTTAAAACATTTTG | Â | Â | Â |