Figure 2

Genomic context of BTC1_14RD and BTC1_130FL. Top panel (A). A modified image from the UCSC cow genome browser (March 2005, Btau_2.0) showing BTC1_14RD and its alternately spliced product BTC1_130FL aligned to the cattle genome (Contig455) [GenBank:AAFC02000448]. Cattle BAC AC146804 [25] aligned to the same region using BLAT (regions of similarity shown with vertical bars). A track for human proteins is shown to demonstrate that there are no known human homologs in this region. A scaled track for cattle ESTs (partial representation of "squish mode" due to the large number of ESTs) shows high support for alternatively spliced cattle transcripts encoded in this region. Bottom panel (B). A modified image from the UCSC human genome browser (May 2004, Hg17) showing the in silico anchoring of BTC1_14RD, represented as a block arrow, to a subtelomeric region of HSA19q on the basis of flanking sequence similarity in cattle BAC AC146804. This region is syntenic to a segment of BTA18 (shown as a separate track at the top). BTC1_130FL anchors in the same region (not shown to maintain clarity of the figure). The assumptive map location of the gene encoding the artiodactyl-specific transcript Ast1 [GenBank:AY427788] is also shown. No significant flanking match was identified in Contig455 (due to its shorter length). Unmodified UCSC Genome Browser tracks for known human proteins, Genscan genes, retroposed genes, conserved sequences, and segmental duplication are shown.