Examples of PCR validation assays for UniformMu FSTs. Each panel shows PCR products that were amplified using a MuTIR primer and a FST locus-specific primer. Each primer was tested on the initial template DNA used to develop the UniformMu FSTs (lane T) as well as DNA extracted from siblings (denoted by brackets). No DNA template controls were included in all assays (lane H2O). W22 DNA was used to confirm that the insertion site did not amplify in the recurrent inbred parent of the UniformMu population.