Co-segregation of pgd3-umu1 with rgh *-00S-005-14. (A) Schematic alignment of the bc199 UniformMu FST site (pgd3-umu1), the TC300585 Zea mays Gene Index EST contig, and the maize assembled genomic island, MAGI4_45584. The schematic shows the location of the MuTIR sequence (black arrow, not drawn to scale) and percent nucleotide identity is noted. The left and right primer sites are marked by gray arrows; primer sizes are not drawn to scale. (B) Co-segregation analysis with progeny from backcross generations 2 and 3 (BC2 and BC3) in a W22 introgression. Left/Right products amplify normal alleles. TIR8/Right products amplify the insertion site. (C) Co-segregation analysis with self progeny. PCR was completed with DNA extracted from homozygous normal (lanes 2–3) or rgh mutants kernels (lanes 4–31).