Sequence-indexed mutations in maize using the UniformMu transposon-tagging population

BMC Genomics

Table 1 Annotations of the insertion sites confirmed in Figures 2 and 3

Blastcluster	MAGI4.0 hit	Insertion site	ZMGI hit	Insertion type	Annotation/Expression Pattern
bc0047	MAGI4_20201	1097	no hit		No EST support for gene
bc0199	MAGI4_45584	731	TC300585	exon	plastid 6-phosphogluconate dehydrogenase
bc0234	MAGI4_4699	748	TC301994	exon	Mixed tissues
bc0472	No hit		TC300035	exon	Probable prefoldin subunit 2
bc0862	MAGI4_29768	394	no hit		No EST support for gene
bc0881	MAGI4_30440	4515	TC290606	exon	Putative ATP dependent copper transporter
bc1133	MAGI4_155851	3281	TC314694	intron	Putative calmodulin-binding protein
bc1266	MAGI4_43305	8436	TC304662	exon	Putative cytosolic chaperonin delta-subunit
bc1474	MAGI4_118260	731	no hit		No EST support for gene
bc1542	MAGI4_139298	550	TC302939	exon	SAM & endosperm expression

A complete table of confirmed insertions is given in Additional file 1 – Annotations of Confirmed Germinal Insertion Sites. The Blastcluster identifiers are from the UniformMu website [49], and correspond to one or more FSTs in Genbank. The Insertion site base number is relative to the matching MAGI4.0 sequence. The Insertion type was classified as exon or intron based on an alignment of the relevant FST, MAGI and ZMGI sequences.

ISSN: 1471-2164