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Figure 4 | BMC Genomics

Figure 4

From: Phase variation and microevolution at homopolymeric tracts in Bordetella pertussis

Figure 4

Representative PCR/LDR data for the BP3651 HPT. (A) Raw capillary electrophoresis data for ligation products from BP3651 uniplex PCR/LDR (green) and molecular weight standards (red) displayed as if an electrophoretic gel image. Strains are indicated above each lane. Ligation products (arrows) are labeled using the notation described in the legend to Figure 1. Letters in teal indicate molecular weight standards as labeled in Additional file 16. (B) Partial traces from direct sequencing of BP3651 PCR products. Strain and alleles are noted above each sequence trace. Purple arrows indicate the point in the sequence trace after which peak shadowing, indicative of a mixture of HPT alleles, can be observed.

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