Figure 5

Representative PCR/LDR data for the tcfA HPT. (A) Raw capillary electrophoresis data for ligation products from tcfA multiplex PCR/LDR (green) and molecular weight standards (red) displayed as if an electrophoretic gel image. Strains are indicated above the lane. Ligation products (arrows) are labeled using the notation described in the legend to Figure 1. Letters in teal indicate molecular weight standards as labeled in Additional file 17. (B) Representative traces from direct sequencing of tcfA PCR products. Strain and alleles are noted above each sequence trace. Purple arrow indicates the point in the sequence trace after which peak shadowing, indicative of a mixture of HPT alleles, can be observed.