STATC is an important regulator of the transcriptional response to hyperosmotic stress. (A) Expected overlap of differentially regulated genes in experiments I and III if STATc is not involved in the transcriptional regulation. (B) Expected overlap in experiments I and II if STATc is the only transcriptional regulator in response to hypertonicity. (C) Venn diagram of the observed differentially regulated genes from the three comparisons: wt cells treated versus untreated (I), RIC cells treated versus STATc- treated (II) and STATc- treated versus untreated (III). The numbers of up- and down-regulated genes of the single experiments are printed in red and green, respectively. Genes shared between 2 or 3 comparisons (shaded region) were applied to further analysis.