Transcriptional profiling of Arabidopsis heat shock proteins and transcription factors reveals extensive overlap between heat and non-heat stress response pathways

Table 1 Overview: Responsiveness of heat shock gene families to abiotic and biotic stress treatments.

Tissue-Treatment	Hsf (n = 21)	Hsp20 (n = 18)	Hsp70 (n = 13)	Hsp90 (n = 6)	Hsp100 (n = 7)
Roots
cold	0.52(0.001)	1.24(< 0.001)	0.45(0.026)	0.32(0.364)	0.25(0.660)
osmotic	0.75(< 0.001)	2.12(< 0.001)	0.48(0.089)	0.74(0.025)	0.66(0.040)
salt	1.17(< 0.001)	1.70(< 0.001)	0.45(0.341)	0.64(0.133)	0.52(0.269)
drought	0.33(0.001)	0.56(< 0.001)	0.26(0.087)	0.15(0.824)	0.20(0.432)
genotoxic	0.24(0.246)	0.72(< 0.001)	0.31(0.061)	0.27(0.258)	0.27(0.243)
oxidative	0.23(0.035)	0.61(< 0.001)	0.26(0.021)	0.19(0.417)	0.31(0.021)
UV-B	0.27(0.050)	0.96(< 0.001)	0.27(0.107)	0.17(0.797)	0.24(0.288)
wounding	0.27(0.043)	1.18(< 0.001)	0.35(0.007)	0.32(0.069)	0.35(0.030)
heat	0.49(0.003)	4.32(< 0.001)	1.55(< 0.001)	1.02(< 0.001)	0.50(0.048)
Shoots
cold	0.62(0.001)	0.51(0.023)	0.44(0.136)	0.57(0.070)	0.35(0.423)
osmotic	0.83(< 0.001)	1.35(< 0.001)	0.76(0.008)	0.46(0.276)	0.65(0.078)
salt	0.52(0.001)	1.00(< 0.001)	0.44(0.017)	0.39(0.116)	0.31(0.275)
drought	0.36(0.002)	0.66(< 0.001)	0.31(0.057)	0.28(0.198)	0.28(0.192)
genotoxic	0.22(0.343)	0.35(0.001)	0.20(0.607)	0.23(0.374)	0.23(0.363)
oxidative	0.29(0.005)	0.78(< 0.001)	0.48(< 0.001)	0.22(0.344)	0.33(0.016)
UV-B	0.50(0.011)	0.69(< 0.001)	0.53(0.024)	0.69(0.020)	0.32(0.499)
wounding	0.33(0.018)	0.62(< 0.001)	0.27(0.225)	0.35(0.099)	0.28(0.248)
heat	0.55(< 0.001)	4.66(< 0.001)	1.34(< 0.001)	1.45(< 0.001)	0.32(0.339)
Leaves
pathogen	0.24(0.701)	0.40(0.096)	0.78(0.001)	0.75(0.018)	0.46(0.134)

The table lists values of the T statistic associated with each tissue-treatment combination with respect to each of five protein families (Hsf, Hsp20, Hsp70, Hsp90, Hsp100). The value of T is proportional to the median level of log₂ fold-change induced by a given stress treatment among the n gene members within a protein family (see Equation 2 in Methods). The P-values associated with each statistic are shown in parentheses. P-values were obtained by genome-wide resampling and represent the probability of obtaining an equal or larger value of T based on 10,000 random samples of n genes from the N = 22746 genes represented on the ATH1 array. P-values exceeding 0.0245 in the table below are non-significant following the Benjamini-Hochberg adjustment for multiple testing (with nominal type I error rate of α = 0.05).

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