Figure 7

The effect of TSA and AzadC on gene expression. L1210 cells were treated without or with TSA (300 nM for 24 h), AzadC (1, 2.5 and 5 μ M for 1, 3 and 5 days), or the combination of TSA and AzadC (1 μ M of AzadC for 1, 3 and 5 days followed by 300 nM of TSA for 24 h). Expression of targets were identified by quantitative RT-PCR. A and B. Zic3 and Tjp1 are methylated and associated with hypoacetyl-H3K9. C and D. Ran and Cog8 are unmethylated and associated with hyperacetyl-H3K9. Each error bar represents the standard deviation calculated from triplicates.