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Table 2 Chromosome locations and putative annotations of the PLUG markers

From: PCR-based landmark unique gene (PLUG) markers effectively assign homoeologous wheat genes to A, B and D genomes

   Wheat chromosome  
Marker no. Type undigested, 1% agarose Hae III-digest, 4% agarose Taq I-digest, 4% agarose Annotation of orthologous rice gene (Pseudomolecules ver. 4)
1 III    3A, 3B, 3D Phospholipase/Carboxylesterase family protein
2 III   3A, 3B 3A, 3B Elicitor-responsive protein 1, putative
3 II 6B 6B 6A, 6B, 6D GTP-binding protein, putative
4 III    6B, 6D expressed protein
5 III   5A, 4D 5A, 4D CIPK-like protein 1, putative
6 III     magnesium transporter CorA-like family protein, putative
7 III     Cysteine synthase, chloroplast precursor, putative
8 III   2A, 2B, 2D 2B RNA recognition motif family protein
9 III    1A, 1B, 1D AML6, putative
10 I 1A, 1B, 1D 1A, 1B, 1D 1A chlorophyll synthase, ChlG family protein
11 III   7A, 7D   senescence-associated protein, putative
12 I 7A, 7B, 7D 7A, 7B, 7D 7A, 7B, 7D Polyprenyl synthetase family protein
13 III    3A, 5A, 5D Phosphatidylinositol N-acetylglucosaminyltransferase subunit A, putative
14 III     COP9 signalosome complex subunit 7, putative
15 III   7A 7B, 7D MSP domain containing protein
16 II 7B   7B expressed protein
17 III    7A BadF/BadG/BcrA/BcrD ATPase family protein
18 II 5A 5A, 5B 5A, 5B, 5D Triosephosphate isomerase, chloroplast precursor, putative
19 II 1A 1B, 1D 1A, 1B, 1D ATP synthase gamma chain, mitochondrial precursor, putative
20 III   1A 1A PRP19/PSO4 homolog, putative
21 III     Ubiquinol-cytochrome c reductase complex 7.8 kDa protein, putative
22 III     Small GTP-binding protein domain containing protein
23 III   5A 5B Aspartyl aminopeptidase, putative
24 II 5B 5A, 5B, 5D 5A, 5B expressed protein
  1. Types I, II and III indicate that 1% agarose gel electrophoresis of PCR products resulted in the separation of three, two, or single bands, respectively. PLUG markers were assigned to chromosomes by electrophoresis on 1% agarose gels, or by electrophoresis of Hae III- or Taq I-digested fragments on 4% agarose gels. The table also shows the annotations of TaEST-LUGs that were used for marker development.