Transcription profiling of SPRN in 21 sheep tissues. (a) RT-PCR with SPRN primers no. 9 and (b) Control PCR with ACTB primers. Marker (M) is the 1 Kb+ ladder (Invitrogen). Samples are cDNA of cerebrum (Cbu), cerebellum (Cbe), obex (Ob), spleen (Sp), kidney (Ki), liver (Li), lung (Lu), heart (He), muscle (Mu), tongue (To), testis (Te), lymph node (Ln), rumen (Ru), omasum (Om), abomasum (Ab), duodenum (Dd), jejunum (Je), ileum (Il), colon (Co), caecum (Ca), rectum (Re), positive control (+) and negative control (-).