Table 2 Amplicon characteristics of primers used for the annotation of the contig and the transcription profiling.
From: Characterization of the genomic region containing the Shadow of Prion Protein (SPRN) gene in sheep
Gene symbol or primer's name | Forward primer (5'-3') Reverse primer (5'-3') | Ta (°C) Length (bp) | Accession number amplicons | % nucleic acid identity/amino acid identity/amino acid positivity with described sequences |
|---|---|---|---|---|
ECHS1 | GCAAAGAATGGGAAAGAACAGCA GGCTCAAAAACCCGCCAGA | 65 646 | EF215853 | OariEST (CD288818): 96/93/94 BtauECHS1 (DQ058603): 96/87/94 HsapECHS1 (NM_004092): 86/80/92 |
PAOX | GCATCTGGACACCTTCTTTGA GTCCTCCCACACCACCTG | 65 274 | EF215855 | OariEST (DY499183): 99/100/100 BtauPAOX (DQ058602): 95/98/100 HsapPAOX (NM_152911): 90/88/94 |
MTG1 | CAGCTACCGCTATCACCGAGGA GCGAGGACTTGCCCACGTT | 60 154 | EF215854 | BtauMTG1 (DQ058604): 92/75/85 |
SPRN | GCGAGGGTGCGTGTGAGG CCTGAGGTCCACGCCCAGTA | 68 236 | - | BtauSPRN (DQ058606): 95/92/93 HsapSPRN (NM_001012508): 79/76/80 |
LOC619207 | GGCTGGTCAACGGCAGCA GGCTCTGTCCCCACGCAGT | 65 220 | EF215856 | HsapLOC619207 (NT_017795): 88/73/84 |
CYP2E1 | AAGAAATTGACAGGGTGATTGG AGGGAAGGAGGTCGATGAAT | 60 117 | EF215857 | OariEST (EE790798): 98/100/100 BtauCYP2E1 (DQ058608): 98/97/100 HsapCYP2E1 (NM_000773): 91/86/94 |
SYCE1 | GACAGCGGCAAGGAGCAGTT GCTTCACATCCTCCAGCTTTGC | 65 149 | EF215858 | BtauSYCE1(NM_001038149): 100/100/100 HsapSYCE1 (NT_017795): 90/90/90 |
OariBAC273H7 UP | GGGACCATCCTGCTGTGACG TCCACTGTCTGCGTCGTCCTC | 65 328 | EI184560 | - |
OariBAC265G4 RP | TGAGAGGTAAGAAGACCACCAAA TCAACCGCAGAACTATGAACC | 63 287 | EI184566 | - |
OariBAC161G10 RP | CAGCCTTGACGCACTCCTTT TTTAGAACTGGGCCACACAGC | 63 200 | EI184563 | - |
ACTB | CGCAGACAGGATGCAGAAAGA GCTGATCCACATCTGCTGGAA | 60 148 | DQ386889 | - |
UP | CGACGTTGTAAAACGACGGCCAG | 55 - | - | - |
RP | CACAGGAAACAGCTATGACCATGATTACG | 55 - | - | - |