Table 3 Amplicon characteristics of the SPRN primers used.
From: Characterization of the genomic region containing the Shadow of Prion Protein (SPRN) gene in sheep
Number SPRN primers | Forward primer (5'-3') Reverse primer (5'-3') | Ta (°C) Length (bp) | Position in sequence DQ870545 |
|---|---|---|---|
1 | ACTCCGGCTCTGGGCTCTGT GGCTCTGTCTTGCTTTCCAAGGT | 63 645 | 1–645 |
2 | TTCAGGGACCACAGGATCGAA CCACGGGCTTCAGCACCTC | 60 487 | 549–1035 |
3 | GTGCGAAGTTGGGGTGAGGA AGCGGGTGAGGGTCTGGAAG | 60 330 | 973–1302 |
4 | GAGGACGGATGCGGTGGAG CCAAAGGAAGCGGGTGAGG | 64 601 | 988–1588 |
5 | CAGGGGTCGCCTCTGGTC CTGCTGGAGGAGTGGGGAGT | 64 507 | 1375–1881 |
6 | ACCCTCACCCGCTTCCTTTG TAGCAGCAGAGCCCAGCACA | 66 486 | 1619–2104 |
7 | CCGCCCCTGAGCCCTGAC CCGCATCCTCCAGGCCAAG | 66 334 | 1967–2300 |
8 | CCGTGTGCTGGGCTCTGCTG GGCGCTCCGTCCTCTGCATC | 68 256 | 2082–2337 |
9 | GCGAGGGTGCGTGTGAGG CCTGAGGTCCACGCCCAGTA | 68 236 | 2189–2424 |
10 | AGCTTGGCCCGGAGGATG CCTGGGTGAGGGTGTTCTGG | 66 678 | 2384–3061 |
11 | AGCCCACCCTGGACACTTGA AGCTGGAGGGAAAAGCACCTG | 66 446 | 2852–3297 |
12 | GGTGCGTCTGTGGATCTGTGAG CTCCCGCTGGTCTTGTGGAG | 66 655 | 3071–3725 |
13 | TCTGGTTGCGGTCAGGGTCT TGAAGTCGGGTTGTTGAGTGGAA | 65 423 | 3574–3996 |
14 | GCCAGATGCCCTCCATCCTC CTGCGAGCACCTTCCAGCTAA | 65 571 | 3760–4330 |
15 | GGAGGGTCGCAACACCACT GGCAGCAGAGTTTATTTCACCAC | 65 279 | 4226–4504 |
16 | CCCGCTTCCAGAATGTGCAG TCCCAGTTCCGTCATGGTCGT | 66 300 | 4263–4544 |
17 | GTGACCTTCCTGCCCTTCAGGTGT GTGTCTGCCCTTCAGCTTCGTGA | 68 250 | 4354–4544 |
18 | TCCCAGTTCCGTCATGGTCGTGTCTGCCCTTCAGCTTCGTGA(T)18 | - | - |