qRT-PCR expression analyses of p53 targets following genotoxic exposure. A) mRNA expression levels of known p53 targets comparing naïve, 7-day NGF (50 ng/mL), 6 h 5-fluorouracil (375 μM) and 6 h bleomycin (10 μg/mL) exposure. Expression was measured via real time PCR and normalized to naïve expression levels using 2-ΔΔct where significance at p ≤ 0.05 is designated by an asterisk (*). Error bars represent standard deviation. B) Same as in Panel A, depicting genes whose expression levels were not regulated in the same manner between NGF treatment and genotoxic treatment. C) Same as in Panel A, depicting genes with comparable regulation between NGF treatment and genotoxic exposure.