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Figure 5 | BMC Genomics

Figure 5

From: Selection of DDX5 as a novel internal control for Q-RT-PCR from microarray data using a block bootstrap re-sampling scheme

Figure 5

Box plots of DDX5 relative expression patterns exhibit small variation across various microarray datasets. The gene expression patterns of DDX5 were obtained from other microarray databases. These datasets were from 84 cancer cell lines (NCI60), which were classified into 8 different cancer cell types and other cell lines (A). GAPDH #5: M33197_3_at; GAPDH #10: 212581_x_at. The box plot results indicated that DDX5 exhibited only small variation across the various NCI60 cell types. For the lung cancer cell lines, DDX5, CLTC and MSN all gave lower variances than ACTB and GAPDH (B). Both ACTB (#1–4) and GAPDH (#5–10) are contained in 10 probe sets on the HG-U133A chip and are shown as #1–10 on x-axis and in Table 1. The variation of DDX5 was also smaller for the HeLa cell cycle dataset, lung cancer dataset and HCC dataset from the Stanford Microarray Database (C). BR: breast cancer, CO: colon cancer, GL: glioblastoma, KI: Kidney, LE: leukemia, LU: lung cancer, ME: melanoma and OV: ovarian cancer.

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