Figure 3

Chromosomal analyses of the Medaka fas, fadd and casp8 genes and their orthologs. (A) A physical map of the region containing the FAS gene was generated for human, chicken and fish. In human and chicken, the FAS gene localizes to a locus on Chr. 10q24.1 or chr. 6. In Medaka, the fas gene resides close to the pten gene in the genome. In the zebrafish genome, the fas-like gene is located between the ptena and ifit2 genes. The fugu fas-like gene is also found near the pten gene. (B) A physical map of the region containing the FADD gene was generated for human, chicken, frog and fish. In human and chicken, the FADD gene localizes to a locus on Chr. 11 or chr. 5. The Medaka fadd gene is located between the tmem16a and npsn genes on chr. 3. In stickleback, the region containing the fadd gene shows a similar synteny. (C) A physical map of the region containing the casp8 gene was generated for Medaka, fugu and stickleback. The casp8 gene localizes between the card-casp8 and mcm6 genes in both Medaka and fugu genomes. In the stickleback genome, the casp8 and card-casp8 genes also localize in tandem. (D) Multialignment of the Medaka Casp8-like molecule (CARD-Casp8) and its homologs identified in fish. The card-casp8 genes from fugu, catfish ([GenBank: AY555576]) and zebrafish were identified by database search. Human ICEBERG ([GenBank: P57730]), which is a CARD-only molecule, was cited for reference. Identical and similar amino acids in all alignments are indicated in black and shaded boxes, respectively. The underline and a box indicate the CARD motif and the protease domain, respectively. Asterisks represent the amino acids essential for catalytic activity.