Figure 4

Molecular phylogenetic analysis of Fas and FADD molecules. (A) A molecular phylogenetic tree of Fas and FADD generated by the neighbor-joining method based on an alignment of death domains. Human, chicken and zebrafish TRADD were used as the outgroup proteins for rooting the tree. The number noted at branches indicates the percentage of times that a node was supported in 1000 bootstrap pseudoreplications and are shown only greater than 50% for the bootstrap value. The scale bar indicates an evolutionary distance of 0.1 amino acid substitutions per position. All of known or identified Fas and FADD proteins in ascidian (Molgula tectiformis), catfish (Ictalurus punctatus), chicken(Gallus gallus), human (Homo sapiens), Medaka (Oryzias latipes), mouse (Mus musculus), stickleback (Gasterosteus aculeatus), West African clawed frog (Xenopus tropicalis) and zebrafish (Danio rerio) were included in this tree. (B) Multialignment of the C-terminus of FADD. Amino acids in a DD are indicated in shaded boxes. A phosphorylated serine residue in human and mouse FADD is indicated by a black box. Analyzed animals: human, mouse, chicken, frog, catfish, stickleback, zebrafish and Medaka.