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Table 1 Functional classification and ratios of genes up-regulated (> 1.5-fold) by exposure to cobalt across all time points

From: Global gene expression profiling in human lung cells exposed to cobalt

Gene ID Gene Name Time (h) Microarray Ratio a qRT-PCR Ratio b
Development, differentiation and proliferation
DNAJA2 DnaJ (Hsp40) homolog, subfamily A, member 2 24 1.94 2.54 *
OAZ1 ornithine decarboxylase antizyme 1 24 1.68 2.26 *
PGF placental growth factor, vascular endothelial growth factor-related protein 0.5 1.50 1.37*
ST13 suppression of tumorigenicity 13 (colon carcinoma) (Hsp70 interacting protein) 24 1.52 1.59 *
TNFRSF9 tumor necrosis factor receptor superfamily, member 9 0.5 1.55 0.54 *
Signal transduction and trafficking
CTNNB1 catenin (cadherin-associated protein), beta 1, 88 kDa 24 1.73  
DLG1 discs, large homolog 1 (Drosophila) 24 3.42 0.38 ***
GNB2L1 guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1 24 1.74 2.64 *
PLA2R1 phospholipase A2 receptor 1, 180 kDa 0.5 1.52  
SCAMP1 secretory carrier membrane protein 1 24 4.00  
SLC12A5 solute carrier family 12, (potassium-chloride transporter) member 5 0.5 1.58 1.69 ***
SLC25A6 solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 6 24 1.89 3.12 ***
SLC2A1 solute carrier family 2 (facilitated glucose transporter), member 1 24 1.85 3.41 *
SLC6A11 solute carrier family 6 (neurotransmitter transporter, GABA), member 11 0.5 1.54  
STXBP1 syntaxin binding protein 1 0.5 1.53  
Cell defense
BNIP3L BCL2/adenovirus E1B 19 kDa interacting protein 3-like 24 1.89 3.30 *
CNIH cornichon homolog (Drosophila) 24 1.62  
IFNA4 interferon, alpha 4 0.5 1.60 0.32 *
MGST1 microsomal glutathione S-transferase 1 24 3.80  
SERPINC1 serine (or cysteine) proteinase inhibitor, clade C (antithrombin), member 1 24 1.59 5.02 *
Protein expression and turn-over
CANX calnexin 24 1.52  
EEF1A1 eukaryotic translation elongation factor 1 alpha 1 24 1.63  
EEF1G eukaryotic translation elongation factor 1 gamma 24 1.76  
EIF4A1 eukaryotic translation initiation factor 4A, isoform 1 24 1.64  
HSPA1A heat shock 70 kDa protein 1A 24 3.22  
HSPCB heat shock 90 kDa protein 1, beta 24 2.21 2.51 *
KIAA1940 KIAA1940 protein 2 2.09  
PPIA peptidylprolyl isomerase A (cyclophilin A) 24 1.59  
RPL15 ribosomal protein L15 24 1.73  
RPL35A ribosomal protein L35a 24 1.68  
RPL36AL ribosomal protein L36a-like 24 1.55  
SERPINH1 serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 1, 24 1.61  
SUI1 putative translation initiation factor 24 2.56  
UBA52 ubiquitin A-52 residue ribosomal protein fusion product 1 4 2.22  
UBB ubiquitin B 24 1.94  
UBC ubiquitin C 24 5.58 8.17 *
WFDC2 WAP four-disulfide core domain 2 24 1.56  
Gene transcription and modification
BHC80 BRAF35/HDAC2 complex (80 kDa) 24 1.76 1.85 *
HOXA9 homeo box A9 0.5 1.50 2.02 *
SDCCAG33 biglycan; serologically defined colon cancer antigen 33 0.5 1.51  
Cell structure and mobility
SDC3 syndecan 3 (N-syndecan) 24 1.62  
Metabolism and energy
AGPAT3 1-acylglycerol-3-phosphate O-acyltransferase 3 24 1.64  
ALDOA aldolase A, fructose-bisphosphate 24 2.75  
GAPD glyceraldehyde-3-phosphate dehydrogenase 24 3.01  
LDHA lactate dehydrogenase A 24 1.66  
OAT ornithine aminotransferase (gyrate atrophy) 24 3.15  
PGK1 phosphoglycerate kinase 1 24 1.77  
SMAP1 stromal membrane-associated protein 1 24 1.65  
TKT transketolase (Wernicke-Korsakoff syndrome) 24 1.86  
Unknown function
GPM6A glycoprotein M6A 2 1.62  
LOC115704   24 1.63  
LTBP3 latent transforming growth factor beta binding protein 3 2 1.71 2.37 *
  1. a: p value < 0.05 with 1.5 fold change.
  2. b: validation with quantitative qRT-PCR (lines in bold) :
  3. *: p value < 0.005, ** p value < 0.01, *** p value < 0.05.
  4. Microarray p-values were calculated using a t-test statistical analysis (Benjamini-Hochberg FDR) on Genespring software. Pair Wise Fixed Reallocation Randomized Test was used to calculate qRT-PCR p values with REST software.
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