Skip to main content

Table 2 Functional classification and ratios of genes down-regulated (< 1.5 fold) by exposure to cobalt across all time points

From: Global gene expression profiling in human lung cells exposed to cobalt

Gene ID Gene Name Time (h) Microarray Ratio a qRT-PCR Ratio b
Development, differentiation and proliferation
NDRG4 NDRG family member 4 24 0.58 0.29 ***
SEZ6L seizure related 6 homolog (mouse)-like 4 0.62  
Signal transduction and trafficking
ATP6V1A ATPase, H+ transporting, lysosomal 70 kDa, V1 subunit A 2 0.66  
AXL AXL receptor tyrosine kinase 4 0.60 1.36 ***
GFRA2 GDNF family receptor alpha 2 0.5 0.63 0.09 *
ICAM1 intercellular adhesion molecule 1 (CD54), human rhinovirus receptor 2 0.60  
IFNAR2 interferon (alpha, beta and omega) receptor 2 24 0.61 0.55 ***
MAPK10 mitogen-activated protein kinase 10 24 0.47 0.31 ***
S 100A 4 S100 calcium binding protein A4 2 0.64  
TFRC transferrin receptor (p90, CD71) 0.5 0.50 0.65 *
Cell defense
BAG1 BCL2-associated athanogene 2 0.65 0.55 **
TNFSF6 tumor necrosis factor (ligand) superfamily, member 6 4 0.54  
Protein expression and turn-over
CNDP2 CNDP dipeptidase 2 (metallopeptidase M20 family) 4 0.57  
FBXL2 F-box and leucine-rich repeat protein 2 0.5 0.55 0.75 ****
TIMP2 tissue inhibitor of metalloproteinase 2 24 0.63 0.54 ***
Gene transcription and modification
MAZ MYC-associated zinc finger protein (purine-binding transcription factor) 2 0.28 0.59 **
NT5E 5'-nucleotidase, ecto (CD73) 24 0.50  
PAI-RBP1 PAI-1 mRNA-binding protein 2 0.62  
SFRS1 splicing factor, arginine/serine-rich 1 (splicing factor 2, alternate splicing factor) 0.5 0.52  
SIN3A SIN3 homolog A, transcriptional regulator (yeast) 24 0.63 2.21 *
Cell structure and mobility
FAT2 FAT tumor suppressor homolog 2 (Drosophila) 4 0.56  
TUBB2 tubulin, beta, 2 4 0.62  
VIL2 villin 2 (ezrin) 0.5 0.64  
Metabolism and energy
AKR1C3 aldo-keto reductase family 1, member C3 24 0.53  
ALDH1A1 aldehyde dehydrogenase 1 family, member A1 24 0.60  
GLCE glucuronyl C5-epimerase 0.5 0.46  
GPD2 glycerol-3-phosphate dehydrogenase 2 (mitochondrial) 4 0.55  
MTHFS 5,10-methenyltetrahydrofolate synthetase 2 0.52  
Unknown function
C20orf30 chromosome 20 open reading frame 30 2 0.48  
C20orf30 chromosome 20 open reading frame 30 24 0.60  
FLJ12806 hypothetical protein FLJ12806 0.5 0.64  
KIAA1582 KIAA1582 protein 24 0.58  
  1. a: p value < 0.05 with 1.5 fold change.
  2. b: validation with quantitative qRT-PCR (lines in bold) :
  3. *: p value < 0.005, ** p value < 0.01, *** p value < 0.05, **** p value < 0.1.
  4. Microarray p-values were calculated using a t-test statistical analysis (Benjamini-Hochberg FDR) on Genespring software. Pair Wise Fixed Reallocation Randomized Test was used to calculate qRT-PCR p values with REST software.