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Figure 1 | BMC Genomics

Figure 1

From: Sensitive and robust gene expression changes in fish exposed to estrogen – a microarray approach

Figure 1

Gene expression changes of VTG, ZP3 and nm23 measured by qPCR and microarray. Hepatic gene expression in rainbow trout of vitellogenin (VTG), zona pellucida protein 3 (ZP3) and a nucleoside diphosphate kinase (nm23) after EE2 exposure measured with qPCR (green bars, male fish) or microarray (blue bars, male fish: red bars, female fish). Values are expressed as fold change (log2) compared to control fish. Paired student's t-tests (single sided) were performed on the qPCR data to confirm/test the putative regulation suggested from microarray data. VTG, ZP3 and nm23 were confirmed to be significantly up-regulated in fish exposed to 10 ng/L (p = 0.001, 0.001 and 0.007 respectively, four biological replicates in each group). ZP3 and nm23, but not VTG were up-regulated in fish exposed to 0.87 ng/L (p = 0.0004, 0.006 and 0.5 respectively, eight biological replicates in each group) in accordance with the microarray data.

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