Table 2 A summary of the four different studies used in the meta-analysis
From: Sensitive and robust gene expression changes in fish exposed to estrogen – a microarray approach
Present study | Hook et al. 2006 | Tilton et al. 2006 | Kishi et al. 2006 | |
|---|---|---|---|---|
Species | O.mykiss | O.mykiss | O.mykiss | O.latipes |
Sex | juvenile | male | juvenile | male |
Estrogenic substance | EE2 | EE2 | E2 | E2 |
Exposure | Water, 10 ng/L | Water, 50 ng/L | Dietary, 5 ppm | Water, 100 ng/L |
Water temperature (C°) | 10 | 12 | 12 | 24 |
Duration (days) | 14 | 7 | 12 | 21 |
Platform | Two-channel spotted cDNA (GRASP 16 k v.1) | Two-channel spotted cDNA (GRASP 16 k v.1) | Two-channel spotted cDNA (GRASP 3.7 k v.1) | One-channel oligonucleotide (60 mer) (Kishi et al. 2006) |
Experimental Setup | Direct comparison, 8 biological replicates | Direct comparison, 3 biological × 3 technical replicates | Reference design, 2 biological × 2 technical replicates | 6 control and 3 exposed biological replicates |
Number of cDNAs/probes | 16006 | 16006 | 3700 | 22587 |
Pre-processing | Loess, no background correction | Loess | Loess | Robust Multichip |
Statistical method used for ranking | Moderated t-statistic | Student t-statistic | fold change | Student t-statistic |
Selected cDNA/probes | 250 (167 induced, 83 suppressed) | 189 (48 induced, 141 suppressed) | 366 (127 induced, 239 suppressed) | 381 (242 induced, 139 suppressed) |
Source for sequences | cDNA, cGRASP | cDNA, cGRASP | cDNA, cGRASP | Transcripts, TIGR (OLGI release 4.0) |
Number of matches (a in D.rerio | 91 | 89 | 190 | 184 |