Table 1 Primer sequences used for gene expression analysis by real time PCR. Amplification was detected by Sybr Green using an Opticon real time PCR machine (BioRad).
Gene | Accession number | Short name | Sequence | Annealing temp used | Product size |
|---|---|---|---|---|---|
HEPCIDIN | BX868476 | Ext1F1 | TGCAGTGGTACTCGTCCTTG | 58 | 167 |
| Â | Â | Ext1R1 | GACGCTTGAACCTGAAATGCTCC | Â | Â |
IRF-1 | AF332147 | IRF-1 F1 | CCGCTGTGCAATGAACT | 60 | 285 |
| Â | Â | IRF-1 R1 | AGGCTGTCTGTGCTGTCTACTAT | Â | Â |
C-TYPE LECTIN | DV191936 | Ext2tF1 | TCTCCTGTCCCATTTTGCTT | 58 | 238 |
| Â | Â | Ext2R1 | GATCCGCCATCCACATATTC | Â | Â |
PRECEREBELLIN | AF192969.2 | Ext3F1 | GCCTTCTCCGCTTCCTTTAT | 58 | 185 |
| Â | Â | Ext3R1 | TTCCCAACATTGCAAGTGAA | Â | Â |
IN NFKbeta | OMY317969 | Exts4F1 | GAAGCCACAGAACATGCTGA | 58 | 242 |
| Â | Â | Exts4R1 | TGGGTGATCACACTGAAGGA | Â | Â |
JUN-B | CF752495.1 | Ext5F1 | TACTGCACTGTTGGGACAGC | 58 | 167 |
| Â | Â | Ext5R1 | CAGAATGCCCCGAGTGTTAT | Â | Â |
LMP2 | AF112117.1 | Ext6F1 | AAGTTCGTTCAGCTGCCACT | 58 | 234 |
| Â | Â | Ext6R1 | GTTGGCAGTCCTCTTTGCTC | Â | Â |
TAP1 | AF115536 | Ext7F1 | CCATGAGTCGCATACACACC | 58 | 188 |
| Â | Â | Ext7R1 | AGTGACCCGCATGAAGTACC | Â | Â |
60S rp L30 | CA037550 | Ext9F1 | GGGATACAAGCAGTCCCAGA | 58 | 211 |
| Â | Â | Ext9R1 | GGGTCGATGATAGCCAGTGT | Â | Â |
CCAAT/EBP | AY144611.1 | Exst10F1 | CAGCGGGTGTTAAGATCCAT | 58 | 200 |
| Â | Â | Exst10R1 | GCAGCAGGAGGATCCAAGTA | Â | Â |
TNF rec | OMY517804 | Ext11F1 | CACCGACTGTGGCAAGTCTA | 58 | 227 |
| Â | Â | Ext11R1 | GGGTCACAGGTAGGCAGTGT | Â | Â |
TAPASIN | DQ092322 | Ext12F1 | GCACGGTGTACTTGCCCTAT | 58 | 188 |
| Â | Â | Ext12R1 | AGGCCCTGTTAACTCCCAGT | Â | Â |
β-ACTIN | AF012125 | RTBAEF | CCAGGCATCAGGGAGTGA | 60 | 283 |
| Â | Â | RTBAER | GTACATGGCAGGGGTGTTGA | Â | Â |
ELF-1α | AF321836.1 | EF1AEF | CAAGGATATCCGTCGTGGCA | 60 | 317 |
| Â | Â | EF1aER | ACAGCGAAACGACCAAGAGG | Â | Â |