Figure 1
Tamoxifen-induced dose dependent and temporal changes in uterine weight. Graphs illustrate fold-change increases in uterine wet (open) and blotted (solid) weight. A) Tamoxifen elicits a dose dependent uterotrophic response (EC50 = 33.7 μg/kg) and achieves maximal induction of approximately 5-fold following three daily doses (3 × 24 hrs) of 100 μg/kg TAM. Significant increases (p < 0.05, n = 5) are denoted by an asterisk (*). In contrast, 100 μg/kg EE (positive control) maximally induced uterine wet weight 11-fold (*, p < 0.05, n = 5) with significant water imbibition (#; p < 0.05, n = 3), while TAM only achieved 50% uterotrophic efficacy and no water imbibition. B) A single dose of 100 μg/kg TAM significantly increased uterine wet weight as early as 24 hrs after administration. No significant water imbibition was observed at any time point.