Figure 4

Overview of the NF2 locus analyzed for point mutations and methylation of CpG dinucleotides. A) Illustration of the NF2 gene displaying exons (black boxes) and conserved non-genic sequences (CNGs) (green boxes) that were analyzed for point mutations. Gene orientation is displayed with an arrow next to the gene name. The scale is according to sequence position on chromosome 22 in million base pairs (Mb) (UCSC Homo sapiens Genome Browser, assembly July 2003). B) Schematic view of the CpG sites in the 5' part of the NF2 gene that were analyzed for methylation. The 40 sequenced CpG sites were located between positions -591 and +166 bp (position +1 refers to transcriptional start site). A single methylated CpG site was detected in tumor M104 at position +120 in ~40% of DNA molecules (displayed as a red filled circle).