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Table 3 Summary of qRT-PCR amplification of cartilage-selective genes in fetal cartilage and seven non-cartilage tissues.

From: Cartilage-selective genes identified in genome-scale analysis of non-cartilage and cartilage gene expression

CV

  

Ct

 

Interval

Symbol

Neg

NC

C

Fold

U133A

     

0–10%

AGC1

2

33

23

371

10–20%

NOS2A1

3

34

23

1158

20–30%

DSPG3

6

35

25

661

30–40%

BDKRB1

2

34

28

49

40–50%

MMP3

3

33

20

4988

U133B

     

10–20%

C10orf49

7

35

23

Unique

20–30%

KIAA1718

 

30

27

8

30–40%

LOC20118

4

34

29

20

40–50%

ASAM

 

31

26

29

U133 2.0

     

10–20%

KIAA0701

 

30

27

5

20–30%

MGC17337

 

30

27

5

30–40%

OSMR

 

28

27

3

40–50%

HIG2

 

30

25

21

  1. Representative genes are listed from each validation platform in order of 10% CV interval. (Neg) Number of non-cartilage tissues in which amplification was not detected. Average Ct values for each gene were calculated for both cartilage (C) and non-cartilage (NC) tissues. Where no amplification was observed the maximum Ct value (i.e. 35) was used for calculations. Fold difference (Fold) is calculated from the difference in cartilage and non-cartilage Ct values.
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BMC Genomics

ISSN: 1471-2164

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