Figure 2

Empirical Cumulative Distributions (ECDs) of standard deviation of the M-values for spikes on loess-normalized data (red line), loess normalized and scaled via the quantile method (green line), loess normalized and scaled via the scale method (black line) and raw data (blue line) on seven technical replicates. The graph shows the frequency (Y-axis) of the standard deviation of the M-values (X-axis). As a term for comparison, we provide the values of the median standard deviations corresponding to the ECDs: red = 0.18, green = 0.19, black = 0.20, blue = 0.36.