Figure 3

Analysis of transcription factors ( ROS-bZIP1 , bHLH-type protein) and other non-regulatory genes (ATP synthase-like protein, auxin-induced protein) that belong to the ' rapid early response ' group (Group-I). (A) Temporal expression profiles by qPCR. Transcript levels at each time point were normalized against the expression of constitutively expressed actin gene. Relative expression of control (0 hour; not shown in the graph) was set to zero by subtracting the values from the relative expression values of each treatment time points. Relative expression values are averages of independent replicates (n = 3; ± SE). The cultivar- specific expression signatures of this group was positively correlated with genotypic variation in chilling tolerance (tolerant – CT6748-8-CA-17; intolerant – INIAP12). Early induction of the ATP synthase-like protein and auxin-induced protein was detected by qPCR but not by microarray. (B) Promoter maps of representative Group-I genes showing the relative position and distribution of known stress-responsive cis-element motifs within the -1,000 bp region. Putative MybR sequences are the prominent group of motifs within the -700 bp region.