Table 3 Gene-specific primers used for quantitative RT-PCR analysis. Primers were designed based on EST and corresponding genomic locus.
Primer name | Sequence (5' to 3') | Locus No. |
|---|---|---|
CAT1-F | CCGTATGGAACAACAACAACTC | Os03g03910 |
CAT1-R | GATACGCTCCCTGTCGAAGT | Â |
bZIP-F | AGCTAAGAGATGCCCTGAATGAAG | Os08g43090 |
bZIP-R | TCTGCGATGACTGTTGTTGCTGTAT | Â |
bHLH-F | CGGGATCGAGCAGGCGGTCAT | Os01g70310 |
bHLH-R | GCAGGAGCACGGTCTTAATTTCTTCAGG | Â |
Myb-F | CCAAGGAGGAGGAGGACACCATC | Os04g43680 |
Myb-R | GCATCGAGGCGCTTCTTGAGG | Â |
HMG-F | GGAAGGCCGGCAAGGACCCCAACA | Os06g51220 |
HMG-R | TTCCACCTATCACCGGCTGCTTTTCCTACA | Â |
ATPSul-F | GCCCAGCTGCGTGAAGAGTTTG | Os03g53230 |
ATPSul-R | AAGAAGGCGTTTGCGTGTATCAGTCA | Â |
Ferri-F | CCCCCAGGCCAAGGACCAGT | Os11g01530 |
Ferri-R | ACGATCAAAGTAGGCGAAAAGGGAGTG | Â |
DI19-F | GGGTGCCCATTTCAGAGTTCA | Os01g73960 |
DI19-R | GCAGATAAATTGGGAGAGCAGTGG | Â |
ATPSyn-F | AGCTCGTGATGAAGTCCATCGTGC | Os12g07140 |
ATPSyn-R | CGTGCTGATGATGACTGCGATGAT | Â |
AUX-F | CAAGAACACGATGGCAACCAACCA | Os01g13030 |
AUX-R | TTACCGGTGCTGAAGCCAATGAAC | Â |
Pfkin-F | CCAAGCCGGGCAGCCTCTCGTT | Os01g47550 |
Pfkin-R | CGCGCCGCCACCGTCTCC | Â |
Ripe-F | CTGTGTTTCGCCTTCGTCTACCATCT | Os04g30490 |
Ripe-R | TCTGCGGGCAAGGACGAACTG | Â |
AnTr-F | TCTCTGAACCCGGATGATATGGACTGC | Os03g05390 |
AnTr-R | CGATGCCCGGAGCGACTTGAT | Â |
ACT2-F | CATGAAGATCAAGGTTGTCGCTCC | Os10g36650 |
ACT2-R | CCAGATTCTTCATACTCAGCCCTTG | Â |
EREBP1-F | CTGCCAAAAGAAAGAGAAAGAACCAAT | Os02g54160 |
EREBP1-R | TGCCAAGCCAGACACGAACAC | Â |