Figure 5From: Monitoring of transcriptional regulation in Pichia pastoris under protein production conditionsIntracellular product retention and BiP development analysed by immunofluorescent staining and flow cytometer analysis during cultivation at two different temperatures. Orange bars: relative fluorescence intensities per cell size obtained at 25 °C steady state; Blue bars: 20 °C steady state. BiP: intracellular signals for the UPR marker BiP/Kar2p (detected with anti-Grp78/BiP specific IgG). HC: intracellular signals for Fab heavy chain (obtained with anti-h-Fab specific IgG); LC: intracellular signals for light chain (analyzed with anti-kappa light chain IgG).Back to article page