From: Monitoring of transcriptional regulation in Pichia pastoris under protein production conditions
Probe name | Gene | Location of the probe in CDS | Probe length (nt) | Tm (°C) | GC% | Probe Sequence 5'-3' | Pool |
---|---|---|---|---|---|---|---|
ACS1 | ERGO:RPPA07570 | 893–917 | 25 | 65.2 | 48.0 | TACTTGGTGGTCAAAAGAGCTCCCA | 1 |
SSE1/2 | ERGO:RPPA10049 | 538–564 | 27 | 63.7 | 44.4 | TTTGACGAGCTTCTCAACTGTCCTGTA | 1 |
VPS17 | ERGO:RPPA07986 | 1583–1611 | 29 | 64.2 | 44.8 | ACCTGCAGACTGGGTGCTAACTTTTTTCT | 1 |
LC | 2F5Fab_light_chain | 409–439 | 31 | 65.6 | 41.9 | GTACTTTGGCCTCTCTGGGATAGAAGTTATT | 1 |
RPL3 | ERGO:RPPA07957 | 489–521 | 33 | 64.9 | 45.5 | TTTTGGTTCAAAGGGGTCTTTCTGATCTGGGTG | 1 |
SEC61 | ERGO:RPPA04132 | 848–882 | 35 | 65.4 | 40.0 | CATGATTGGCATGTTGGATGTGTAGAACAATCTGA | 1 |
PDI1 | ERGO:RPPA04694 | 768–804 | 37 | 64.3 | 40.5 | GGCTAAAGGGATGTTAGCTTCAGCATATGATTTGAAG | 1 |
TRR1 | ERGO:RPPA06201 | 815–853 | 39 | 66.4 | 46.2 | CGAAAACACCTGGAATAGATGTAAGGGAAGAACCTGGGA | 1 |
RIB1 | ERGO:RPPA08084 | 756–796 | 41 | 66 | 39.0 | AAGAACGTTTGTCTGCTGGGTGTCTTAGTAAAAGATTTGCT | 1 |
TKL1 | ERGO:RPPA06932 | 1782–1824 | 43 | 67.5 | 39.5 | TATTGGAACACCGTCTGGAAGGACTGATAATTGGTAAGATCTA | 1 |
ARG1 | ERGO:RPPA07954 | 813–857 | 45 | 70.1 | 44.4 | GTCAAACCTGGAGTTTCATAACAACCTCTGGACTTGATTCCGATG | 1 |
SNZ3/2 | ERGO:RPPA10229 | 557–581 | 25 | 63.3 | 44.0 | ATGGCAGCAGCTATCTTTTCAGGAT | 2 |
PMT6 | ERGO:RPPA04967 | 1054–1080 | 27 | 62.8 | 44.4 | GTAATCTGTTGTTGGCTGGAACCTTGA | 2 |
GCN4 | ERGO:RPPA07905 | 359–387 | 29 | 67.1 | 48.3 | AACGGCTGGAGTGGTAGCAAGAGTAGTTT | 2 |
SSA4 | ERGO:RPPA10651 | 1605–1635 | 31 | 65.9 | 45.2 | CATATGATTCCAAGCCATTCTTGGCAGCAAC | 2 |
HC | 2F5Fab_heavy_chain | 669–701 | 33 | 67.9 | 48.5 | GATTTGGGCTCAACTTTCTTGTCCACCTTGGTG | 2 |
KAR2 | ERGO:RPPA06939 | 1325–1359 | 35 | 65 | 40.0 | AGTTGGGATAGCAGTGTTTCTGTTGATTAAGGTAG | 2 |
SEC53 | ERGO:RPPA08162 | 349–385 | 37 | 63.8 | 40.5 | CGTTTCTGAACTCAATGAATGTTCCTCTTCTGATTGG | 2 |
IMH1 | ERGO:RPPA04985 | 1949–1987 | 39 | 62.4 | 38.5 | TGTCTTTTTCCAATTCATCTCGTTTGGAGAGGACTAAAG | 2 |
BFR2 | ERGO:RPPA04523 | 700–740 | 41 | 65.8 | 39.0 | AACTCTTGGCTCACAATCTTGCTTTTGTTTAATAGCTTGCC | 2 |
SEC31 | ERGO:RPPA06211 | 1243–1285 | 43 | 70 | 44.2 | TTTCGTCGACAAGAGTTTGGTAATCGTTTGTGCTGATGGTGCT | 2 |
HSP90/82 | ERGO:RPPA05876 | 891–915 | 25 | 63.9 | 48.0 | CTTAACAGCCAATGGGTCTTCCCAA | 3 |
ARO4 | ERGO:RPPA09892 | 786–812 | 27 | 62.8 | 44.4 | TTTCCGTGGGAACAGTCAATCATCAGA | 3 |
ERO1 | ERGO:RPPA06115 | 1531–1559 | 29 | 64.4 | 44.8 | TCTCGGTGCCTTTGAGTGCCAATGAAAAT | 3 |
TAL1 | ERGO:RPPA08309 | 550–580 | 31 | 63.5 | 41.9 | CCTTGTACCAGTCAAGAATACGTCCAACAAA | 3 |
BMH2 | ERGO:RPPA07190 | 546–578 | 33 | 66.1 | 48.5 | AAATGACAGGCGCGGTCAGGAGAGTTTAGAATC | 3 |
ROT2 | ERGO:RPPA08267 | 1634–1668 | 35 | 63.3 | 40.0 | ATACGACCTAGTAAGAACAAATGGCCTATGGTTTG | 3 |
GLR1 | ERGO:RPPA07699 | 965–1001 | 37 | 67.7 | 45.9 | CTGTTAGATAGTTTTCTACCAGCAGCGATTGCGACAG | 3 |
RAD16 | ERGO:RPPA09210 | 1007–1045 | 39 | 63.6 | 38.5 | TATTGTGGGCTTCGTCTAAAATGACCCTATAAAAATGCG | 3 |
GSH2 | ERGO:RPPA06484 | 949–989 | 41 | 61.8 | 39.0 | AGAATTTGTTGAACTTTTTTACAACCGCTAAGCTGGGTCAG | 3 |
RAD2 | ERGO:RPPA08434 | 361–403 | 43 | 65.8 | 39.5 | CTTGGGTAACCTCATCCGAATCTCTTTGGTCTTTTTTATGTTG | 3 |
CNE1 | ERGO:RPPA04260 | 749–793 | 45 | 69.9 | 42.2 | GGATGTACAATTCAGCATCTTCGTTCCAGTTTTCTGGTTTCACAG | 3 |
HOG1 | ERGO:RPPA06104 | 581–605 | 25 | 67.8 | 56.0 | TCATCTCGGCGAAAATGCACCCTGC | 4 |
SEC18 | ERGO:RPPA05138 | 1027–1053 | 27 | 63.5 | 40.7 | TACCAATATCCAGCTTGTTGGTACCAA | 4 |
CUP5 | ERGO:RPPA08088 | 123–151 | 29 | 63.3 | 41.4 | CACTGTATTCTTGATCAGTAAGTCTGGAC | 4 |
SAR1 | ERGO:RPPA08441 | 475–505 | 31 | 62.9 | 38.7 | CCTGTCTTAAGTAGATAGAGCAGGTAAATAC | 4 |
ARL3 | ERGO:RPPA09566 | 336–368 | 33 | 62.5 | 42.4 | AACGTCCTGTTTATTGGCAAGCATAAGAATCGG | 4 |
DFM1 | ERGO:RPPA10235 | 824–860 | 37 | 63.1 | 40.5 | CATTTCTTATCGCAGTAGCTTGGTTTTTCCGTCTTTG | 4 |
HEM12 | ERGO:RPPA08255 | 816–852 | 37 | 63.1 | 40.5 | TGGACGGAATAACCAATCCAACGAGACAACATCATAA | 4 |
RAD54 | ERGO:RPPA04321 | 2193–2231 | 39 | 63.9 | 38.5 | ACACATGAACTCAATTGCAGTTTAGCTGATTGTCTTTGG | 4 |
RPS23B | ERGO:RPPA09269 | 187–227 | 41 | 66.2 | 41.5 | AACTGAACTCTGACACACTTTCTAATAGCGGAGTTAGGTTG | 4 |
MSN2 | ERGO:RPPA05326 | 985–1027 | 43 | 70.5 | 41.9 | GCTCATTCGTGTCTATGGACTTTGCCCTCTTTCTTTGTTTTGT | 4 |
SSO2 | ERGO:RPPA06663 | 356–400 | 45 | 71.4 | 40.0 | GCTGTTCTCTGTAGTTGCTTTCAATAATACGGTAGTCCTGAATAG | 4 |
GLT1 | ERGO:RPPA07339 | 1740–1764 | 25 | 63.4 | 48.0 | CACAATCGATTCCCTGATGGGATCA | 5 |
UBC1 | ERGO:RPPA09581 | 273–299 | 27 | 64.4 | 44.4 | AGAATAGGCGTCCAGGCATTCTTCAAT | 5 |
HSP12 | ERGO:RPPA05227 | 211–239 | 29 | 64.8 | 44.8 | TGAACTTGCTCAACTAAGGTAGGTTGGGT | 5 |
PFK1 | ERGO:RPPA04164 | 2447–2479 | 33 | 63.4 | 39.4 | TTTTACCCGATTTGTTCTTACCATCGTCTTCCT | 5 |
CIT1 | ERGO:RPPA10025 | 758–792 | 35 | 67.1 | 40.0 | GATAGACAGGTATAATCTCATCAGCTCAACGAACT | 5 |
HRD1 | ERGO:RPPA05158 | 1188–1222 | 35 | 63.8 | 42.9 | TGAAGGAATTATCGGAATTGGACTGCAGTGGGAAT | 5 |
SNC1 | ERGO:RPPA04520 | 275–313 | 39 | 63.9 | 41.0 | AGGAACGATGATAACAATCAGCAAAATCACGATTCCCAG | 5 |
KIN2 | ERGO:RPPA04639 | 640–680 | 41 | 70.6 | 43.9 | TTTACGGTTGCATCGTAGTCATCAAATTCTGTCCCGTCAAG | 5 |
TSA1 | ERGO:RPPA06990 | 540–582 | 43 | 66.5 | 44.2 | GGACTTGGAAAAGAATTCCTTGGAAGCATCAACTTCTGGCTTG | 5 |
CPR5 | ERGO:RPPA09912 | 314–358 | 45 | 71.9 | 42.2 | TGAAGTTCTCGTCCTTGAATCTGCTACCGTAGATAGATTTTCCTC | 5 |