Experimental validation of novel genes predicted in the un-annotated regions of the Arabidopsis genome

BMC Genomics

Table 2 Success rate and structural characteristics for RACE-targeted intergenic genes.

Target Statistics	Twinscan	EuGene	Combined
Number targeted	726	623	1071*
Mean CDS length	456 bp	354 bp	-
Median CDS length	357 bp	264 bp	-
Average # of exons	2.0	1.4	-
% single exon predictions	423 (58%)	452 (73%)	-
Experimental Statistics	Twinscan	EuGene	Combined
Number successful^†	304 (42%)	257 (41%)	378 (35%)
Mean CDS length	445 bp	452 bp	441 bp
Median CDS length	339 bp	330 bp	321 bp
Average # of exons	1.8	1.7	1.7

* Primer pairs were designed for a total of 1071 total loci. 623 pairs of primers were compatible with a EuGene prediction, and 726 pairs of primers were compatible with a Twinscan prediction. 278 primer pairs were compatible with both a Twinscan and a EuGene prediction.
† For Twinscan and EuGene, success rates are defined as the number of FL sequences obtained that overlap a Twinscan or EuGene prediction, as compared to the number targeted. For the Combined category, the success rate represents the total number of loci verified with respect to the total number targeted.

ISSN: 1471-2164