Table 1 The 50 most abundant transcripts, revealed by SAGE analysis, in S. mansoni adult worms.
From: A quantitative view of the transcriptome of Schistosoma mansoni adult-worms using SAGE
Gene name | Tag sequence | Number of tags | Gene/cluster identification – Accession number | Position inside transcript | Tag rank | |
|---|---|---|---|---|---|---|
1 | S.mansoni eggshell protein (F10) gene | actattcggg | 1454 | gi| 160993| gb| M14309.1| SCMFSPA | 50 | 0 |
2 | S. mansoni pol-polyprotein | cctgtaaact | 835 | gi| 44829167| tpg| DAA04497.1| C200397.1 | 99 | 0 |
3 | S.mansoni heat shock protein 86 mRNA | gaagaagtgg | 640 | gi| 161027| gb| J04017.1| SCMHSP86 | 96 | 0 |
4 | S. mansoni 14-3-3 protein (Sm14-3-3) mRNA | tcatacaaga | 588 | gi| 790657| gb| U24281.1| SMU24281 | 95 | 0 |
5 | Similar to S. japonicum L23a ribosomal protein | ccggtgtctc | 513 | TC13660 | 97 | 0 |
6 | No matches | agcgtccaaa | 472 | TC7406 | 85 | 0 |
7 | S. mansoni heat shock protein 70 (HSP70) gene | aggcaagtgg | 464 | gi| 161025| gb| L02415.1| SCMHSP70X | 67 | 0 |
8 | S. mansoni (GAPDH) | cataatgaag | 438 | gi| 160994| gb| M92359.1| SCMGAPDH | 89 | 1 |
9 | S. mansoni fructose bisphosphate aldolase mRNA | cggctcagga | 398 | gi| 2598925| gb| AF026805.1| AF026805 | 72 | 0 |
10 | Mitochondrial small subunit ribosomal RNA | gtagtgcttg | 364 | AF130787_1459_201 | 94 | 0 |
11 | No matches | cttttctaaa | 349 | TC17835 | 93 | 0 |
12 | Similar to S. japonicum SJCHGC02196 protein | tgttgttcgt | 340 | TC17184 | 96 | 0 |
13 | S.mansoni (Liberia) mRNA for tandem repeat | agcaatggaa | 319 | gi| 454257| emb| Z29960.1| SMTANREP TC7340 | 20 | 3 |
14 | Similar to SJCHGC06305 protein (putative piruvate kinase) | tgtacgtcat | 313 | gi| 56757978| gb| AAW27129.1| TC7454 | 94 | 2 |
15 | S. mansoni fatty acid binding protein mRNA | tatcgttcta | 302 | gi| 160983| gb| M60895.1| SCMFABP14 | 89 | 0 |
16 | Similar to unknown protein S. japonicum (putative S3a ribosomal protein) | gcgagtcgaa | 293 | gi| 56758226| gb| AAW27253.1| TC16783 | 86 | 0 |
17 | Similar to S. japonicum SJCHGC00690 protein. Putative beta thymosin | taatatgcgc | 265 | gi| 76161984| gb| AAX30141.2| TC11200 | 71 | 0 |
18 | Similar to S. japonicum Sj-Ts1 | gtgctcgaag | 255 | gi| 14581393| gb| AAF98445.1| TC17397 | 53 | 0 |
19 | S. mansoni 28 kDa glutathione S-transferase (GST) gene | tgactgatct | 254 | gi| 161010| gb| M98271.1| SCMGSTM | 88 | 1 |
20 | S. mansoni tegumental protein Sm 20.8 mRNA | gcacattgtc | 252 | gi| 2454222| gb| U91941.1| U91941 | 62 | 0 |
21 | S. mansoni actin mRNA | acatcaacaa | 225 | gi| 924602| gb| U19945.1| SMU19945 | 84 | 0 |
22 | similar S. japonicum (putative ribosomal protein L15) | ccttcggtac | 225 | gi| 29841092| gb| AAP06105.1| TC13745 | 97 | 0 |
23 | Similar to SJCHGC09089 S. japonicum (putative ribosomal protein S10) | gaggttatgg | 215 | gi| 56755876| gb| AAW26116.1| TC16990 | 89 | 0 |
24 | Similar to S. japonicum clone ZZD545 mRNA sequence | ttggaggcaa | 211 | gi| 28317769| gb| AY223294.1| TC13521 | 93 | 0 |
25 | S.mansoni SM22.6 antigen (A12) RNA | gagaacacca | 198 | gi| 161086| gb| M37003.1| SCMSM226 | 71 | 0 |
26 | Similar to SJCHGC01209 S. japonicum (putative tetraspanin) | gtaaccaatg | 193 | gi| 56752993| gb| AAW24708.1| TC11060 | 93 | 0 |
27 | Similar to S. japonicum (putative hnRNP A2) | cagcgtcctt | 191 | gi| 29841163| gb| AAP06176.1| TC10489 | 78 | 3 |
28 | Similar to SJCHGC06078 S. japonicum (putative 40S ribosomal protein) | gggattgccg | 190 | gi| 56758252| gb| AAW27266.1| TC16808 | 87 | 0 |
29 | S. mansoni receptor for activated PKC mRNA | gtctgctgat | 186 | gi| 19071248| gb| AF422164.1| | 87 | 0 |
30 | No matches | ccaggttgtg | 184 | TC9014 | 0 | 0 |
31 | Similar to S. japonicum HEXBP (putative DNA binding protein) | gttatggcca | 174 | gi| 29841170| gb| AAP06183.1| TC10354 | 75 | 0 |
32 | Similar to S.japonicum SJCHGC05715 (putative germinal Histone H4) | tggattcttg | 168 | gi| 56754980| gb| AY813941.1| TC14578 | 81 | 0 |
33 | S. mansoni myosin heavy chain (MYH) mRNA | gtacttagtg | 167 | gi| 161043| gb| L01634.1| SCMMYH | 97 | 0 |
34 | S. mansoni lactate dehydrogenase | tatgttctct | 165 | gi| 4099443| gb| U87629.1| SMU87629 | 87 | 0 |
35 | NADH dehydrogenase 4 (ND4) gene | attttgtttg | 159 | AF130788_1003_2262 | 87 | 0 |
36 | S. mansoni cathepsin L | gggtatgaat | 159 | gi| 473158| emb| Z32529.1| SMCATHL | 87 | 2 |
37 | Similar to S. japonicum (putative 60S acidic ribosomal protein P0) | ggattcggtt | 155 | gi| 29841185| gb| AAP06198.1| TC7332 | 95 | 0 |
38 | S. mansoni cDNA clone (putative 60S acidic ribosomal protein P2) | ccatcagcct | 154 | gi| 34701209| gb| CD164545.1| CD164545 CD164545 | 92 | 0 |
39 | Similar to S. japonicum putative L10 ribosomal protein | gccccttgga | 154 | TC13587 | 87 | 0 |
40 | S. mansoni enolase trans-spliced mRNA | tcgttctgat | 147 | gi| 1002615| gb| U30175.1| SMU30175 | 84 | 0 |
41 | Non-available | tccccgtaca | 145 | no_annot | ||
42 | S. mansoni cysteine protease inhibitor (Cys) mRNA | cccaccactt | 144 | gi| 33355622| gb| AY334553.1| | 84 | 0 |
43 | Similar to S. japonicum cDNA clone (putative ribosomal protein L10) | tacctaggcc | 143 | gi| 29841379| gb| AAP06411.1| TC7615 | 79 | 0 |
44 | Similar to S. japonicum SJCHGC01239 protein (putative L8 ribosomal protein) | gtcgcaaagt | 139 | gi| 56754665| gb| AAW25518.1| TC7475 | 59 | 1 |
45 | S. mansoni NADH dehydrogenase subunit 5 (NU5M) mRNA | ggtttagtag | 138 | gi| 3599492| gb| AF085145.1| AF085145 | 90 | 0 |
46 | Non-available | tgcgcgcgtg | 137 | no_annot | ||
47 | Similar to S. japonicum SJCHGC02603 Protein (putative 40S ribosomal protein S27) | cacagacagc | 134 | gi| 60687866| gb| AAX30266.1| TC8025 | 55 | 0 |
48 | S. mansoni clone NLSL20 40S rRNA protein homolog mRNA | tggtgagggc | 130 | gi| 2623827| gb| AF030961.1| AF030961 TC13698 | 46 | 0 |
49 | Similar to S. japonicum SJCHGC01998 protein (putative 40S ribosomal protein S25) | ctctcgagga | 128 | gi| 76162217| gb| ABA40776.1| TC7478 | 70 | 0 |
50 | Similar to S. japonicum SJCHGC00821 protein (putative myosin regulatory light chain 2A) | ggagaagaaa | 123 | gi| 56757579| gb| AAW26951.1| TC7368 | 3 | 3 |