Table 2 Transcripts with putative alternative poly-adenylation events in S. mansoni, as suggested by SAGE.
From: A quantitative view of the transcriptome of Schistosoma mansoni adult-worms using SAGE
Gene (Accession GenBank) | mRNA size (nt) | CDS | SAGE tag sequence; tag position (nt); tag frequency (tags/million); tag rank. | Use of canonical polyadenylation site? (Sequence & Position – nt) | Affecting coding region?# | AREs possibly removed by the alternative poly-adenylation event* |
|---|---|---|---|---|---|---|
Rac GTPase (AY158217) | 2,383 | 211..777 | tgtgtgtgta; 951; 366; 1. | No | No | 3 out of 4 |
acaagttatg; 1959; 351; 0. | No | No | ||||
Receptor tyrosine kinase (AF101194) | 6,022 | 120..4799 | tcaatcatta; 821; 249; 1. | No | Yes | 4 out of 4 |
aagaaatgca; 2013; 132; 0. | No | No | ||||
Myosin light chain (AF071011) | 965 | 30..512 | aatcctaatc; 651; 29; 1. | No | No | 2 out of 2 |
aatatataca; 819; 44; 0. | Yes | No | ||||
Calponin homolog (U86674) | 1,963 | 12..1097 | tttatcttca; 1467; 44; 1. | Yes (AATAAA – 1,480) | No | 2 out of 4 |
cccaaccctc; 1677; 513; 0. | No | No | ||||
Dynein light chain (U55992) | 469 | 6..275 | gcattgtata; 163; 29; 1. | No | No | 0 out of 0 |
aaacccataa; 207; 689; 0. | No | No | ||||
Enolase trans-spliced (U30175) | 1,461 | 40..1344 | caacgttggt; 650; 29; 1. | Yes (ATTAAA – 1,024) | Yes | 2 out of 2 |
tcgttctgat; 1235; 2154; 0. | Yes (AATAAA – 1,353) | No | ||||
Cyclophilin (L46884) | 571 | 16..501 | tgtcagggtg; 189; 44; 1. | Yes (AATAAA – 250) | Yes | 0 out of 0 |
ttgttttcgg; 382; 1465; 0. | No | No | ||||
Actin (U19945) | 1,538 | 45..1175 | gccgacgagg; 44; 29; 2. | No | Yes | 5 out of 5 |
aagtgtgatg; 893; 1216; 1. | No | Yes | 5 out of 5 | |||
acatcaacaa; 1307; 3297; 0. | Yes (AATAAA – 1,366) | No | ||||
Phosphofructokinase (L31531) | 3,087 | 147..2492 | ttcctttcat; 2895; 29; 2. | No | No | 1 out of 3 |
ttttccgttt; 2984; 59; 1. | Yes (AATAAA – 3,025) | No | 0 out of 3 | |||
taaaaaaaaa; 3043; 103; 0. | No | No | ||||
Triose phosphate isomerase (M83294) | 1,080 | 33..794 | atgtcgatgg; 714; 29; 1. | Yes (ATTAAA – 749) | No | 4 out of 4 |
tcagttactt; 844; 938; 0. | Yes (AATAAA – 1,066) | No | ||||
Superoxide dismutase (M27529) | 623 | 7..561 | gataccccag; 319; 29; 2. | No | No | 0 out of 0 |
tgctacaata; 530; 29; 1. | No | No | 0 out of 0 | |||
aaatgatttt; 557; 249; 0. | Yes (AATAAA – 588) | No | ||||
Cu/Zn superoxide dismutase (M97298) | 605 | 23..484 | cctattctcc; 513; 498; 1. | No | No | 2 out of 2 |
cacaaataaa; 580; 161; 0. | Yes (AATAAA – 588) | No | ||||
PUR-alpha-like (AF254148) | 1,282 | 77..880 | tgcttaatag; 1110; 191; 1. | No | No | 1 out of 3 |
ttagatttct; 1242; 15; 0. | No | No |