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Table 2 Transcripts with putative alternative poly-adenylation events in S. mansoni, as suggested by SAGE.

From: A quantitative view of the transcriptome of Schistosoma mansoni adult-worms using SAGE

Gene (Accession GenBank) mRNA size (nt) CDS SAGE tag sequence; tag position (nt); tag frequency (tags/million); tag rank. Use of canonical polyadenylation site? (Sequence & Position – nt) Affecting coding region?# AREs possibly removed by the alternative poly-adenylation event*
Rac GTPase (AY158217) 2,383 211..777 tgtgtgtgta; 951; 366; 1. No No 3 out of 4
    acaagttatg; 1959; 351; 0. No No  
Receptor tyrosine kinase (AF101194) 6,022 120..4799 tcaatcatta; 821; 249; 1. No Yes 4 out of 4
    aagaaatgca; 2013; 132; 0. No No  
Myosin light chain (AF071011) 965 30..512 aatcctaatc; 651; 29; 1. No No 2 out of 2
    aatatataca; 819; 44; 0. Yes No  
Calponin homolog (U86674) 1,963 12..1097 tttatcttca; 1467; 44; 1. Yes (AATAAA – 1,480) No 2 out of 4
    cccaaccctc; 1677; 513; 0. No No  
Dynein light chain (U55992) 469 6..275 gcattgtata; 163; 29; 1. No No 0 out of 0
    aaacccataa; 207; 689; 0. No No  
Enolase trans-spliced (U30175) 1,461 40..1344 caacgttggt; 650; 29; 1. Yes (ATTAAA – 1,024) Yes 2 out of 2
    tcgttctgat; 1235; 2154; 0. Yes (AATAAA – 1,353) No  
Cyclophilin (L46884) 571 16..501 tgtcagggtg; 189; 44; 1. Yes (AATAAA – 250) Yes 0 out of 0
    ttgttttcgg; 382; 1465; 0. No No  
Actin (U19945) 1,538 45..1175 gccgacgagg; 44; 29; 2. No Yes 5 out of 5
    aagtgtgatg; 893; 1216; 1. No Yes 5 out of 5
    acatcaacaa; 1307; 3297; 0. Yes (AATAAA – 1,366) No  
Phosphofructokinase (L31531) 3,087 147..2492 ttcctttcat; 2895; 29; 2. No No 1 out of 3
    ttttccgttt; 2984; 59; 1. Yes (AATAAA – 3,025) No 0 out of 3
    taaaaaaaaa; 3043; 103; 0. No No  
Triose phosphate isomerase (M83294) 1,080 33..794 atgtcgatgg; 714; 29; 1. Yes (ATTAAA – 749) No 4 out of 4
    tcagttactt; 844; 938; 0. Yes (AATAAA – 1,066) No  
Superoxide dismutase (M27529) 623 7..561 gataccccag; 319; 29; 2. No No 0 out of 0
    tgctacaata; 530; 29; 1. No No 0 out of 0
    aaatgatttt; 557; 249; 0. Yes (AATAAA – 588) No  
Cu/Zn superoxide dismutase (M97298) 605 23..484 cctattctcc; 513; 498; 1. No No 2 out of 2
    cacaaataaa; 580; 161; 0. Yes (AATAAA – 588) No  
PUR-alpha-like (AF254148) 1,282 77..880 tgcttaatag; 1110; 191; 1. No No 1 out of 3
    ttagatttct; 1242; 15; 0. No No  
  1. Transcripts with alternative poly-adenylation events were considered only when confirmed by at least 2 SAGE tags, without downstream internal binding sites for oligo-dT (fake polyA). # – alternative-polyadenylation events affecting the coding region were considered when the alternative upstream tags were located at least 256 nt before the original stop-codon. CDS – stands for the cDNA coordinates of the coding region of the gene. * refers to the number of putative Adenylate Rich Elements (AREs) present in the 3'UTR, that are located downstream of the most 5' SAGE tag found in a transcript, out of the total 3' UTR AREs of the transcript.