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Figure 2 | BMC Genomics

Figure 2

From: The dystrotelin, dystrophin and dystrobrevin superfamily: new paralogues and old isoforms

Figure 2

Alternative splicing and alternative promoter usage. A) Conservation of alternative splicing of dystrophin and α-dystrobrevin across vertebrates. Mammalian alternative splicing [11, 27–29, 33] and exon numbering [33, 81] are as published. Our data show alternative splicing of dystrophin exons 73 and 78 and α-dystrobrevin exons 9 and 13 in fish. Green boxes – coding regions in each isoform, yellow boxes – skipped coding exons, white boxes, 3'-UTR. B) Developmental profile of dystrophin alternative splicing in zebrafish, showing a steady increase in transcripts lacking exon 73 and a defined peak of transcripts lacking exon 78 early in development. Results are means of three experiments, ± SD. C) Conservation of alternative internal promoter use in the vertebrate dystrophin and utrophin genes. The schematic diagram shows the positions of alternative first exons for the Dp260, Dp140, Dp116 and Dp71 dystrophin isoforms and for G-utrophin, all previously characterised in mammals. Our findings show that Dp260 and Dp140 are conserved across tetrapods and that Dp116, Dp71 and G-utrophin are conserved across all gnathostomes. For each isoform, green boxes show sequence which is protein-coding in both that short isoform and the full-length protein, yellow ones show sequence which is coding in only one of these two, and white shows non-coding sequence. Numbers at top give exon numbers according to the human dystrophin gene [81]. Novel frog and fish sequences are presented separately [see Additional File 2].

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