Figure 4

Preliminary characterisation of mouse dystrotelin. A) Expression profile of mouse dystrotelin by semi-quantitative RT-PCR of RNA from dissected mouse tissues. GAPDH RT-PCR is given as an RNA loading control. B) In situ hybridisation of a dystrotelin cRNA probe to whole-mount mouse embryo (E10.5), showing staining of the central nervous system. C) Confocal fluorescent microscopy of a mouse dystrotelin-EGFP fusion construct heterologously expressed in COS7 cells (arrow-heads, cytoplasmic membrane; arrows, perinuclear staining), compared with the largely nuclear staining observed with the empty EGFP vector. Scale bar 10 μm.