Figure 1

Multiple sequence alignment of predicted ACE3 sequences from human, mouse, rat, cow and dog with human testicular ACE. HEXXH motif indicated. Underlined are the predicted N-terminal signal sequences (human and rat ACE3) and C-terminal transmembrane regions (human, rat, mouse and cow ACE3), along with the known N-terminal signal sequence and C-terminal transmembrane region in testicular ACE. The human ACE3 sequence was restored by compensating for ^fbase deletion(s)/insertions and suppressing *stop codons. Positions of the forward and reverse RT-PCR primers are indicated by the arrows beneath the sequence. Accession numbers: Mouse XP_110936; Rat XP_573208; Dog XP_548034; Cow XP_600103.