Figure 1

Estimation of the size of the 25 zebrafish LG chromosome pairs using flow cytometry. (A) A flow karyogram depicting the mixed chromosome preparation of human and zebrafish chromosomes. Flow-peaks contained in the oval represent the zebrafish chromosomes (see B for the chromosomal composition of each peak). The largest zebrafish LG chromosomes (5 and 7) were slightly larger than human chromosome 18 (76.12 Mb) and the smallest zebrafish LG chromosomes (22 and 25) were similar to the size of human chromosome 21 (46.94 Mb). Thus, human chromosomes 18 and 21 were chosen as references (labeled in the karyogram). (B) A flow karyogram of the zebrafish chromosome preparation. LG chromosomes ranged in size from 42.2 to 77.9 Mb and the LG chromosomes composing each peak are denoted. The similarity of size resulted in only LG chromosomes 3, 4, and 24 separating into distinct peaks. The remaining nine peaks contained 2 to 5 chromosomes.