Figure 1

Quantitative precision of DArT-BSA. For each marker, there is a 1:1 relationship between the relative hybridization contrast (= log₂ [cy3/cy5] between bulks as a percentage of log₂ [cy3/cy5] between parents) and the allele-frequency difference between the bulks. This relationship makes interpretation of experimental results straightforward. The plot is based on a comparison of mPub bulks (size = 40), prepared from genomic representations of individual Steptoe/Morex DH plants (see corresponding genome scan in Figure 3). It includes all markers present in a previously published Steptoe/Morex DArT map [20] and reports the difference in the frequency of Steptoe alleles between bulks.