Figure 2

Schematic representation of Pdcd2 and Tbp transcripts in mouse testes. 1) to 4): forms of Tbp transcripts; 1) constitutive Tbp transcript, 2) alternative Tbp transcript not reaching Pdcd2, 3) Pdcd2as1 transcript overlapping the entire alternative Pdcd2 exon, 4) Pdcd2as2 transcript, the longest Tbp alternative, overlapping the entire Pdcd2 gene, 5)Updcd2 – two putative transcripts upstream of Pdcd2 using the same promoter as Pdcd2. Numbered arrows represent the primers used for real-time PCR. Primer pair 1–2 detects both Pdcd2 transcripts, 3–4 just constitutive Pdcd2, 3–5 just alternative Pdcd2, 6–7 Pdcd2as2, 8–9 both antisense mRNAs (Pdcd2as1 and Pdcd2as2), 10–11 all forms of Tbp. For other legend see Fig. 1.