Figure 2

Confirmation of NAT expression by strand-specific RT-PCR. A) Schematic illustration of the primer orientation for the strand-specific RT-PCR; left panel: for transcripts of the category "overlap"; right panel: transcripts of the category "antisense". B) RT-PCR products of transcripts of the category "overlap" with indicated product sizes. C) RT-PCR products of transcripts of the category "antisense". D) Strand-specific RT-PCR for three transcripts with no prior evidence of SAS pairing. Sense: RT reaction with 3' reverse primer; antisense: RT reaction with 5' forward primer; -primer: no primer during RT reaction; H₂O: no template control.