Figure 4

Detection of MYCN gene amplification in neuroblastoma cell lines in CGP. A. Confirmation of MYCN amplification in neuroblastoma cell lines by Southern hybridization. B. CGP analysis of neuroblastoma cell lines. DNA copy number profiles for chromosome region 2p25.3-2q14.3 (approximately every 1.3 Mbps) containing the MYCN gene were derived from 96 oligonucleotide primers. All 12 neuroblastoma-derived cell lines were examined, but only the SH-SY5Y, TNB-1, NGP and SK-N-BE lines are displayed. Horizontal axis indicates each locus from 2pter (bp), and vertical axis shows test/reference log₂ fluorescence ratio. The MYCN loci are indicated by black circles. C. CGP high-resolution analysis (approximately every 48 kbps) in neuroblastoma cell lines. DNA copy number profiles for chromosome 2p24.2-2p24.3 containing the MYCN gene were derived from 72 oligonucleotide primers and represented 66 loci. All 12 neuroblastoma-derived cell lines were examined, but only NBL-S, SK-N-DZ, TGW and RTBM1 cell lines are displayed. Horizontal axis indicates each locus from 2pter, and vertical axis shows test/reference log₂ fluorescence ratio. The arrow and black circles denote the MYCN loci. D. Summary of the amplified region surrounding the MYCN gene. The vertical axis indicates the amplified loci between 15.5 Mega (M) bp and 17.5 M bp from 2pter. The black bars denote the DNA amplification sites for each cell line. Regions where amplifications were detected in more than 2 spots of the CGP assay were defined as "amplification sites". The dashed line represents the loci of NAG, DDX1, MYCN and FAM49A.