Figure 1

Evaluation of RNA samples from human liver and hepatocytes. A. RT-PCR was employed to evaluate the quality of RNA samples from human hepatocytes (right) by detecting the transcripts of albumin (ALB) and cytoskeleton protein 19 (CK19), where human liver (left) was used as reference and H₂O was employed as control. Each PCR was performed using 35 thermal cycles and then the PCR products were observed by electrophoresis on 2% agarose gels. B. 16 genes with less than 3 TPM were randomly selected for the evaluation of those MPSS signatures with low frequency via RT-PCR, where β-actin and H₂O were employed as internal and negative controls, respectively. Each PCR was generally performed using 35 thermal cycles and then the PCR products were observed by electrophoresis on 2% agarose gels. The numbers indicate the TPM of the MPSS signatures.