Differential gene expression in femoral bone from red junglefowl and domestic chicken, differing for bone phenotypic traits

Table 5 Taq-man qPCR-assay details

Gene name	Ensembl transcript ID¹	GenBank ID²	Forward primer	Reverse primer	Reporter Probe
WDR5	ENSGALT00000004189	[GenBank:CN225644]	CTCTTAAGCTTTGGGACTACAGCAA	AGTACTTCTCATTTTTGTGTCCTGTGT	TCTTCAGGCACTTTCC
PTK1	ENSGALT00000026060 ENSGALT00000026061 ENSGALT00000030798	[GenBank:CN232734]	TCCGCCAGAGGAGTACGT	GCGACTCATCCACTGTTGCTA	CAAGCCAACCTCCTTTACC
TGOLN2	ENSGALT00000023308	[GenBank:CN223571]	CGGCGGCCCAAATCTG	GCAGCTTTCTTCCACTGAATACCT	CCTGGATCAAAAGATCTAG
WIF1	ENSGALT00000016042	[GenBank:BU128191]	CGGGATTCGAAGGAGACCAAT	TCTTACCCATACATTTTCCTCCATTTCG	ATGGCATTTACTGATTTCAC
GAPDH	ENSGALT00000014280 ENSGALT00000023323	[GenBank:CN233267]	GGAGTCCACTGGTGTCTTCAC	GCTGAGATGATAACACGCTTAGCA	CCCTTCAGATGAGCCCCAG
EDIL3	ENSGALT00000025199 ENSGALT00000025200 ENSGALT00000025201	[GenBank:CN224434]	CCTACAAGCTTGCCTACAGTAATGA	ACATTCTTCCGGTGTGTGTCAT	CCTGGAAAACCTTGTCCTTC
CDCA8	ENSGALT00000000432 ENSGALT00000008916	[GenBank:CN226240]	GTCTTCATTACCGTTCCTGTTGGA	CCTCCGGATTGAGATGAAGAAGATT	CTGTTAAACGAATGCTCTCCC
LIMS1	ENSGALT00000027130	[GenBank:CN228750]	GCAGTTTCCTGAAGGGCTCTT	ACCACACTGATGGCAGCAA	TTCCTCCCTTCAAACTCAT
NCE2	ENSGALT00000006045	[GenBank:CN227630]	GCTGGGCTCCAACTAGAACATT	CAAAGGATCGTCAAAATTCAGAAGATCAG	CCCCAGACAACATCCTTT
NDUFA7	ENSGALT00000000895	[GenBank:CN225183]	CGGCTCCGCAACTTCCT	GCTGCGTCCTCTTGGAGATC	CTGGAGGTCCCGCCCC

¹ Transcripts targeted by each Taq-man assay. Specified are Ensembl transcript IDs.
² GenBank ID of probe for which assay was designed.

ISSN: 1471-2164