TY - JOUR AU - Barrasa, M. Inmaculada AU - Vaglio, Philippe AU - Cavasino, Fabien AU - Jacotot, Laurent AU - Walhout, Albertha JM PY - 2007 DA - 2007/01/18 TI - EDGEdb: a transcription factor-DNA Interaction database for the analysis of C. elegans differential gene expression JO - BMC Genomics SP - 21 VL - 8 IS - 1 AB - Transcription regulatory networks are composed of protein-DNA interactions between transcription factors and their target genes. A long-term goal in genome biology is to map protein-DNA interaction networks of all regulatory regions in a genome of interest. Both transcription factor -and gene-centered methods can be used to systematically identify such interactions. We use high-throughput yeast one-hybrid assays as a gene-centered method to identify protein-DNA interactions between regulatory sequences (e.g. gene promoters) and transcription factors in the nematode Caenorhabditis elegans. We have already mapped several hundred protein-DNA interactions and analyzed the transcriptional consequences of some by examining differential gene expression of targets in the presence or absence of an upstream regulator. The rapidly increasing amount of protein-DNA interaction data at a genome scale requires a database that facilitates efficient data storage, retrieval and integration. SN - 1471-2164 UR - https://doi.org/10.1186/1471-2164-8-21 DO - 10.1186/1471-2164-8-21 ID - Barrasa2007 ER -