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Table 2 Comparison of expression patterns (fold change) observed with microarray versus qRT-PCR analysis

From: Transcriptome profiling of the small intestinal epithelium in germfree versus conventional piglets

Gene symbol

Gene expression with qRT-PCR1

Microarray analysis

 

GF

CONV

Ratio

P value

Ratio

FDR adjusted P value

NFκBIA

1.00 ± 0.21

1.81 ± 0.21

1.81

0.05

1.79

P > 0.05

TOLLIP

1.00 ± 0.08

1.69 ± 0.12

1.69

0.01

0.97

P > 0.05

IRF7

1.00 ± 0.55

3.09 ± 0.63

3.09

0.05

0.83

P > 0.05

IFNAR1

0.73 ± 0.22

1.56 ± 0.22

2.14

0.04

1.53

0.013

IFNGR1

1.00 ± 0.32

2.62 ± 0.46

2.62

0.05

1.52

0.030

LIFR

0.52 ± 0.54

1.42 ± 0.63

2.73

0.07

2.31

0.003

STAT1

0.98 ± 0.28

2.09 ± 0.28

2.13

0.03

1.86

0.001

STAT2

1.00 ± 0.18

3.05 ± 0.20

3.05

0.03

1.01

P > 0.05

pan SLA I

1.42 ± 0.73

4.53 ± 0.85

3.19

0.04

2.44

0.001

B2M

1.13 ± 0.36

5.17 ± 0.42

4.58

0.01

3.97

0.009

  1. 1Values are means ± SE, n = 3/4 per treatment group. RNA was isolated from ileal sample of GF versus CONV piglets using Trizol reagent. Quantitative RT-PCR analysis of gene expression was determined by QuantiTect SYBR Green RT-PCR Master Mix in a 384-well plate using an ABI Prism 7900 HT SS instrument.
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BMC Genomics

ISSN: 1471-2164

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