Figure 2From: Sampling Daphnia's expressed genes: preservation, expansion and invention of crustacean genes with reference to insect genomesThe distribution of gene annotations for the list of 787 Daphnia pulex ESTs based on results from Blastx searches against the NCBI non-redundant protein database. (A) The assignment of 799 annotations of biological process to 227 EST clusters from level 4 of the Gene Ontology. (B) The assignment of 371 annotations of molecular function to 288 EST clusters from level 3 of the Gene Ontology. Blastx queries recorded the best 5 matches with an E-value threshold of 1 × 10-3 and a minimal value of 33 aligned amino acids. Gene Ontology (GO) terms were assigned to ESTs using Blast2GO [22] with the following configurations: Pre-eValue-hit filter 1 × 10-3; Pre-similarity-hit filter 2; Annotation cut-off 35; GO weight 5.Back to article page