The distribution of gene annotations for the list of 787 Daphnia pulex ESTs based on results from Blastx searches against the NCBI non-redundant protein database. (A) The assignment of 799 annotations of biological process to 227 EST clusters from level 4 of the Gene Ontology. (B) The assignment of 371 annotations of molecular function to 288 EST clusters from level 3 of the Gene Ontology. Blastx queries recorded the best 5 matches with an E-value threshold of 1 × 10-3 and a minimal value of 33 aligned amino acids. Gene Ontology (GO) terms were assigned to ESTs using Blast2GO  with the following configurations: Pre-eValue-hit filter 1 × 10-3; Pre-similarity-hit filter 2; Annotation cut-off 35; GO weight 5.