Table 1 Representative Bvg+ phase specific genes identified by microarray analysis.
Function | Gene | Product | Fold-Change ± SEM |
|---|---|---|---|
Cell Adhesin | BB0110 | putative adhesion | 8.58 ± 3.21 |
|  | BB1658 fim3 | serotype 3 fimbrial subunit precursor | 32.47 ± 11.10 |
|  | BB1936 fhaL | adhesion | 8.04 ± 1.63 |
|  | BB2312 fhaS | adhesion | 10.37 ± 2.76 |
|  | BB2989 fimD | fimbrial adhesion | 11.58 ± 3.96 |
|  | BB2992 fimA | fimbrial protein | 33.75 ± 13.88 |
|  | BB2993 fhaB | filamentous hemagglutin | 11.37 ± 4.55 |
|  | BB3424 | fimbrial protein | 8.92 ± 2.64 |
|  | BB3425 fimN | fimbrial subunit protein | 11.95 ± 2.06 |
|  | BB3426 fimX | fimbrial protein | 8.92 ± 2.64 |
|  | BB3674 fim2 | serotype 2 fimbrial subunit precursor | 21.89 ± 11.68 |
Cell Envelope | BB1289 | putative integral membrane protein | 26.29 ± 8.25 |
|  | BB1368 | putative membrane protein | 3.94 ± 0.56 |
|  | BB3119 | putative membrane protein | 19.27 ± 2.17 |
|  | BB3157 | putative membrane protein | 16.41 ± 5.54 |
|  | BB4029 | putative glycosyl transferase | 4.96 ± 0.32 |
|  | BB4266 | putative membrane protein | 6.84 ± 0.82 |
|  | BB4269 | putative glycosyl transferase | 2.23 ± 0.42 |
|  | BB4284 | putative membrane protein | 2.96 ± 0.14 |
Protein Folding and Ushering | BB2320 dsbG | thiol:disulfide interchange protein precursor | 35.29 ± 9.68 |
|  | BB2991 fimB | chaperone protein | 11.20 ± 7.71 |
|  | BB3749 mucD | serine protease; trypsin activity | 2.75 ± 0.41 |
|  | BB3803 | putative peptidyl-prolyl cis-trans isomerase | 4.65 ± 1.26 |
Global Regulatory Functions | BB0308 | putative transcriptional regulator | 5.67 ± 0.98 |
|  | BB1607 | putative LysR-family transcriptional regulator | 3.37 ± 0.55 |
|  | BB1638 brpL | putative RNA polymerase sigma factor | 50.66 ± 15.58 |
|  | BB1642 | putative regulator; catalytic activity | 112.16 ± 97.97 |
|  | BB1645 | putative anti-sigma factor; ATP binding | 9.46 ± 3.00 |
|  | BB1646 | putative antisigma factor antagonist | 18.19 ± 6.08 |
|  | BB2051 | putative regulatory protein | 2.24 ± 0.59 |
|  | BB4374 | putative AsnC-family transcriptional regulator | 5.78 ± 7.25 |
Metabolism | BB0457 | probable enoyl-CoA hydrates; isomerase activity | 5.72 ± 1.34 |
|  | BB0458 | probable carboxymuconolactone decarboxylase | 3.42 ± 0.79 |
|  | BB1476 | putative gluconate dehydrogenase | 2.88 ± 0.51 |
|  | BB1478 | putative carbonic anhydrase precursor | 4.05 ± 0.77 |
|  | BB1640 | hypothetical protein; glutamate-cysteine ligase activity | 63.92 ± 18.15 |
|  | BB1644 alr | alanine racemase, catabolic | 253.96 ± 97.47 |
|  | BB2658 | glutamate decarboxylase | 2.78 ± 0.93 |
|  | BB3783 | intracellular PHB depolymerase | 3.23 ± 0.78 |
|  | BB4286 nadC | putative nicotinate-nucleotidepyrophosphorylase | 32.86 ± 6.48 |
Periplasmic, Exported, Lipoprotein | BB0324 cyaA | bifunctional hemolysin-adenylatecyclase precursor | 6.73 ± 2.52 |
|  | BB1280 | putative exported protein | 73.44 ± 37.80 |
|  | BB1292 | putative exported protein | 272.88 ± 93.88 |
|  | BB1641 | putative exported protein | 149.05 ± 97.51 |
|  | BB2398 | putative exported protein | 6.77 ± 1.38 |
|  | BB2873 | putative membrane protein | 26.85 ± 13.90 |
|  | BB3068 | putative exported protein | 10.90 ± 3.11 |
|  | BB3242 | putative exported protein | 48.75 ± 9.65 |
|  | BB3302 | lipoprotein | 5.09 ± 0.63 |
|  | BB3978 dnt | dermonecrotic toxin | 8.63 ± 3.61 |
|  | BB4181 | putative lipoprotein | 3.40 ± 0.25 |
|  | BB4285 | putative exported protein | 49.73 ± 14.04 |
Transport | BB0323 cyaC | cyclolysin-activating lysine-acyltransferase | 2.70 ± 0.27 |
|  | BB0325 cyaB | cyclolysin secretion ATP-binding protein; ABC transporter | 2.10 ± 0.31 |
|  | BB0326 cyaD | cyclolysin secretion protein; transporter activity | 3.48 ± 0.51 |
|  | BB0327 cyaE | cyclolysin secretion protein; subtilase activity | 3.66 ± 1.57 |
|  | BB0419 sphB1 | autotransporter subtilisn-like protease; substilase activity | 59.34 ± 43.26 |
|  | BB1593 | putative ABC transport system, membrane protein | 83.60 ± 28.88 |
|  | BB1643 | putative inner membrane transport protein | 39.61 ± 16.68 |
|  | BB1864 vag8 | autotransporter | 122.00 ± 79.14 |
|  | BB2270 | autotransporter | 4.71 ± 2.07 |
|  | BB3826 bfrD | probable TonB-dependent receptor for iron transport | 2.68 ± 0.58 |
|  | BB3993 ompQ | outer membrane porin protein OmpQ | 24.34 ± 8.07 |
TTSS | BB1616 bopN | putative outer protein N | 504.05 ± 189.58 |
|  | BB1620 bopD | putative outer protein D | 457.16 ± 172.40 |
|  | BB1624 bscI | putative type III secretion protein | 226.35 ± 80.58 |
|  | BB1628 bscN | putative ATP synthase in type III secretion | 115.85 ± 37.00 |
|  | BB4228 bopCa | hypothetical protein; characterized type III effector | 15.70 ± 3.85 |