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Table 3 Fold-changes identified by quantitative real-time PCR.

From: Construction and validation of a first-generation Bordetella bronchiseptica long-oligonucleotide microarray by transcriptional profiling the Bvg regulon

Gene

Product

qRT-PCR

Microarray

BB0057 rpoA

DNA-directed RNA polymerase alpha chain

-2.03 ± 0.01

-1.09 ± 0.06

BB0110

putative adhesion

92.47 ± 0.35

8.58 ± 1.21

BB0376

putative membrane protein

6.23 ± 0.45

3.00 ± 0.72

BB0419 sphB1

autotransporter subtilisin-like protease

137.34 ± 6.96

59.34 ± 4.26

BB1037

LysR-family transcriptional regulator

-1.24 ± 0.01

-1.07 ± 0.15

BB1315

putative universal stress protein

-64.15 ± 0.35

-11.43 ± 0.03

BB1593

putative ABC transport system, membrane protein

1544.59 ± 8.49

83.60 ± 8.88

BB1619 bcrH1

putative regulatory protein

2943.53 ± 16.33

340.10 ± 7.39

BB1622 bcrH2

putative regulatory protein

1532.48 ± 11.25

286.50 ± 9.03

BB2108

probable two-component response regulator

-39.21 ± 1.24

-18.08 ± 0.01

BB2147 hemN

oxygen-independent coproporphyrinogen III oxidase

-23.10 ± 3.33

-5.84 ± 0.01

BB2320 dsbG

thiol:disulfide interchange protein precursor

358.29 ± 5.25

35.29 ± 1.68

BB2323

putative transcriptional regulator

-21.86 ± 3.46

-11.03 ± 0.01

BB2367

putative regulatory protein; transcription factor activity

-11.71 ± 2.15

-2.95 ± 0.11

BB2434 pckG

phosphoenolpyruvate carboxykinase (GTP)

-12.57 ± 1.46

-2.88 ± 0.04

BB2435

S-adenosylmethionine-dependent methyltransferase

-5.10 ± 1.24

-2.76 ± 0.04

BB2522

conserved hypothetical protein

-58.35 ± 0.35

-2.82 ± 0.03

BB3447 cysN

sulfate adenylyltransferase subunit 1; (ATP) activity

-12.50 ± 0.15

-2.00 ± 0.25

BB3703 eno

enolase; phosphopyruvate hydratase activity

-2.68 ± 0.14

-1.11 ± 0.08

BB4495

probable ATP-binding component of ABC-transporter

-30.63 ± 0.15

-33.12 ± 0.01

BB4506 rpoN

probable sigma (54) modulation protein

-11.66 ± 0.56

-3.38 ± 0.12

BB4835 rpoH

RNA polymerase sigma-32 factor

-6.33 ± 0.22

-3.09 ± 0.11

BB4989 dnaA

chromosomal replication initiator protein

1.94 ± 0.01

1.27 ± 0.19

  1. Quantitative real-time PCR analysis was used to measure mRNA levels present in B. bronchiseptica RB50 compared to mRNA levels present in B. bronchiseptica RB54. Differences in mRNA levels detected by both qRT-PCR and Microarray are listed as mean fold-changes ± standard deviation. qRT-PCR fold-changes were calculated using the relative quantitative (ΔΔCT) method, as detailed in the Methods, from three biological sample sets.