Figure 5

Reduced anti-oxidation via Sod3 in males compared with females. A: Gender difference in ROS was examined in glomeruli of 36 day-old Mhm rats, perfused with heparin in PBS or with PBS alone. The elevation of ROS in males is shown on the y-axis (expressed as the ratio of male vs. female). In PBS-perfused kidneys, the elevation of superoxide anion in males was statistically significant. This statistical significance diminished when kidneys were flushed with heparin. Also the elevation of total ROS in males attenuated (the ratio of male vs. female became smaller). B: Real-time PCR confirmed significant under-expression of Sod3 in males compared with females (p < 0.01).